Differential interference contrast is now the standard for many professional life science applications. This method achieves aesthetic microscope images with the highest resolution and a very high contrast . As a contrast method, DIC generates impressive optical cuts , and is particularly useful for uncoloured, unfixed samples and living organisms that cannot be compressed. With preparations such as diatoms or radiolaria, it is possible to obtain aesthetic, textbook-quality images .
The DIC set works according to the Nomarski method. The light is first polarized and divided into two parallel but spatially separated beam paths by a special prism in the condenser. Depending on the material properties or light refraction, interference occurs between these two parallel beam paths when they pass through the sample. This accentuates certain structures in a pseudo 3D relief. Both beam paths are combined in the DIC slider, which is located behind the objectives; the rotatable polarizing filter then enables the desired contrast effect or the direction of the relief effect to be set and perfectly matched to the sample.
In combination with a Nexcope NE900 series microscope, this contrast method provides you with a professional observation instrument for your scientific investigations . It also provides an affordable option for amateur enthusiasts. With the Nexcope DIC, you receive a complete, pre-configured set that you can use without any prior specialist knowledge . Unlike phase contrast, adjustments are not required during the installation process. The DIC set is ready for use in a few simple steps, and the S-Apo objectives can also be used for bright field, dark field or polarization applications. While observing with the NE900 series microscope, you can switch directly between DIC, bright field, polarization or epi-fluorescence.
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